Ghrita and Taila kalpana are important preparations which use the process of incorporating drugs in oily particle to target them to their site of action. The Jatyadi Taila and Jatyadi Ghrita are important preparations which are used very commonly in Ayurvedic field of surgery as Vrana Ropak (wound healer). This study explores the rationality behind the Paka Kala, Murcchana and its utility with chemical analysis and comparative antimicrobial study of Jatyadi Taila & Jatyadi Ghrita. Jatyadi Taila and Jatyadi Ghrita has an effective antimicrobial activity against Staphylococcus and Pseudomonas in both Diffusion methods (Kirby- Bauer disc diffusion method and Stokes disc diffusion method) and Dilution methods (Broth dilution method & Agar dilution method). The Acid value, Saponification value, Peroxide value, Ester value, thin Layer Chromatography of two samples was evaluated.